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DIG-RNA labelling

Rafal Butowt butowt at scs.unr.edu
Wed Apr 22 20:11:17 EST 1998

After in vitro transcription my 2.2 kb DIG-RNA is not extracted good
during phenol/chloroform (probably is going to organic phase). But my
non-labelled 2.2 kb RNA is extracted good in the same condition (phenol is
good equilibrated/pH is OK).
Does anyone had similar problems? Any suggestions?
Thank You

Dep Biochemistry
Univ of Nevada, Reno
89557-0014  Reno

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