RNA from cell culture

JOSE LUIS CARRASCO JIMENEZ jlcarrasco at ibmcp.upv.es
Fri Apr 24 11:00:35 EST 1998


  Dear colleagues,

  I am having some problems to isolate good quality RNA from
tobacco cell culture. Do you know of any modification
to standard RNA isolation protocols which might be useful
when starting from cell cultures? Apparently, I am dealing
with polysaccharide contamination, since the samples are
highly dense and viscous.

  Any hint, advice or comment will be highly appreciated.

  Thanks in advance.

  Best wishes,

  Jose L. Carrasco
  jlcarrasco at ibmcp.upv.es






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