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SSLP analysis of C24 ecotype

HAUSER MARIE-THERES hauser at edv2.boku.ac.at
Fri Aug 14 11:13:44 EST 1998


Dear netters,

We have performed SSLP analysis with DNA from  Landsberg
erecta and C24 and separated the PCR fragments on 5% native PAA
gels. Our results are summarized in the following table with
approximatley band sizes.

Chromsome      Marker            C24            Ler
I               modified ateat1        152 bp        152bp
I              nga248                      129 bp        129 bp
I              nga280                      85 bp            85 bp
I              nga111                      180 bp         162 bp
I              nga63                        110 bp           89 bp
I              nga59                        115 bp         115 bp
I              athatpase                    69 bp           69 bp

II            nga1126                     210 bp         199 bp
II            nga361                       100 bp         120 bp
II            Athbio2                     140 bp         209 bp
II            nga168                       135 bp         135 bp

III          nga172                       150 bp          136 bp
III          nga162                       130 bp           89 bp
III          Athcdc2BG               127 bp          129 bp
III          nga6                           150 bp          123 bp

IV          nga8                           198 bp          198 bp

V           nga225                        119 bp          119 bp
                                          (different from published)
V          nga151                        150 bp          120 bp
V          nga139                        132 bp          132 bp
V          nga76                          280 bp         > 200 bp
V        modified  nga129          100 bp          100 bp
V         Athso191                     150 bp          156 bp
V         nga249                          115 bp          115 bp
V         nga106                          130 bp          123 bp
V         Athctr1A                       138bp          143 bp

For the CAPS marker AG on Chromosome IV we could find a polymorphism
between Ler and C24 with the enzyme HinfI.

If there are any questions we are pleased to help you.

Tomas Kocabek  (kocabek at umbr.cas.cz)
 and
Marie-Theres Hauser




----------------

Dr. Marie-Theres Hauser

Center of Applied Genetics
Universitaet fuer Bodenkultur Wien
Muthgasse 18
A-1190 Vienna
Austria/EUROPE

Tel: +43-1-36006-6371 or -6374
FAX: +43-1-36006-6392
e-mail: hauser at mail.boku.ac.at
web page: http://www.boku.ac.at/zag/




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