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Fixing of Arabidopsis before GUS staining

Paul Rushton rushton at mpiz-koeln.mpg.de
Mon Dec 7 11:32:18 EST 1998


Does anyone know how to fix Arabidopsis material before GUS staining. My
problem is that some of our promoter-GUS constructs show massive
wound-induced expression. The normal method of GUS staining does not kill
the cells quickly enough to stop this wound response and therefore further
investigation of the expression patterns are difficult.

Thanks a lot

Dr Paul Rushton
Max-Planck-Institut fuer Zuechtungsforschung
Cologne
Germany

rushton at mpiz-koeln.mpg.de




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