depleting ATP pool

Dmitry Belostotsky dab at cnsvax.albany.edu
Wed Jul 1 20:03:59 EST 1998


Dear Arabidians,

I am wondering if it is possible to quickly and significantly reduce the
intracellular ATP pool in Arabidopsis and/or tobacco BY-2 suspension
culture cells. We want to starve an ATP-dependent enzyme before cell
lysis and protein isolation, so the artefacts and inability of cells to
metabolically recover dont matter.

Thanks in advance for your suggestions
DB
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Dmitry A. Belostotsky
Assistant Professor

Department of Biological Sciences
State University of New York at Albany
1400 Washington Ave.
Albany NY 12222
Tel. (518) 442 4368
FAX  (518) 442 4767
E-mail dab at cnsvax.albany.edu
http://barbara.bio.albany.edu/~dab/
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