Reference for Arabidopsis transformation by infiltration

Jan Lucht lucht at fmi.ch
Mon Jul 20 11:29:13 EST 1998


Dear Netters,

I have been using the "floral dip" method for Arabidopsis transformation
by Agrobacterium-infiltration without vacuum since the first rumors
about it appeared in this newsgroup, but never found a literature
reference for it although the method is widely used.

Now there is a literature reference describing it:

Bent, A. F., and S. J. Clough. "Agrobacterium germ-line transformation:
Transformation of Arabidopsis without tissue culture." In: Plant
Molecular Biology Manual (Second Edition), S. B. Gelvin and R. A.
Schilperoort, eds. (Kluwer Academic Pub., Dordrecht, The Netherlands).
1998, section B7, pages 1-14

The authors also provide a short protocol on their homepage:

http://www.cropsci.uiuc.edu/~a-bent/protocol.html

Infiltration without vacuum really saves time and effort, and the
transformation efficiencies in my hands are very similar with both
methods.

Jan Lucht

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