internal standard for RT-PCR

daemon at net.bio.net daemon at net.bio.net
Thu Jul 23 17:38:35 EST 1998


Hi everybody,
Does anybody know a good candidate gene to serve as internal standard in
RT PCR on Arabidopsis RNA. It has to fulfill these two requirements:
constitutively expressed in all organs and at every stage of
development, and very low expression (my gene of interest is itself low
expressed and I want to co-amplify the standard and the target). Any
advice is welcome.
Thank you very much.

--
Nicolas Baumberger
Institute of Plant Biology, University of Z=FCrich
Zollikerstr.107, CH- 8008 ZURICH, Switzerland
Tel: 41-1-6348232
=46ax: 41-1-6348257
Email: nbaumber at botinst.unizh.ch





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