New activation tagging T-DNA lines available from ABRC and NASC

daemon at net.bio.net daemon at net.bio.net
Mon Jun 8 14:02:28 EST 1998


The Arabidopsis Biological Resource Center (ABRC) and Nottingham
Arabidopsis Stock Centre (NASC) are pleased to announce that 8,550
new T-DNA lines are now available for distribution.  These lines
include an activation tagging construction so that screens for
expression effects can be conducted. This population is sent
mainly as pools of 100 lines, although a set of smaller pools is
availabe.  Below is a general description of these lines,
including price and ordering information.

Please contact Dr. Randy Scholl, ABRC or Dr. Mary Anderson, NASC
at the addresses below if you have questions.

------------------------------

Activation tagged T-DNA pools from Dr. Detlef Weigel

Dr. Detlef Weigel, The Salk Institute for Biological Sciences, La
Jolla, California generated activation-tag T-DNA lines.
Initially, 850 of these lines were donated directly by Dr. Weigel,
and a subsequent 7,700 were grown to distribution-level quantities
by Dr. Michael Sussman, Dept. of Horticulture, University of
Wisconsin, Madison.

The background for this population is Columbia.  The pSKI15 vector
used in the transformations contains an activation tag construct,
a pBstKS+ plasmid rescue sequence and a phosphonothricin
resistance gene (BAR driven by 5=92 MAS) between the T-DNA left and
right borders. The activation-tag construction is comprised of
multimerized 35S  Cauliflower Mosaic Virus (CaMV) enhancers.
Plasmid rescue can be conducted in conjuction with either the
right or left T-DNA borders.  See the Weigel web site
(http://www.salk.edu/LABS/pbio-w/index.html) for details of the
rescue procedure and the sequence of the T-DNA.  The T-DNA
sequence is also available on the AIMS site.

The distributed T-DNA pools are T4 seeds.

The seed pools of these lines are distributed primarily as pools
of 100 lines. For the first 850 lines, pools of 10 and single
lines are available.  The remaining 7,700 lines are available only
as pools of 20.  The single lines are distributed on a limited
basis, by special arrangement and fee.  Please contact the Center
for details.  Stock numbers and costs for sets of pools are:

Stock #,  Description...................... ABRC Price/NASC Price

CS21995/N21995,  86 pools of 100 (1st + 2nd donations)...$100/70 L

CS21999/N21999,  Set of 85 pools of 10 lines (1st
	donation, 850 lines)................Contact centers for price

CS21998/N21998,  Set of 385 pools of 20 lines
	(2nd donation, 7,700 lines).........Contact centers for price

NOTE: All sets of pools include Columbia wild type.

-------------------------

Stock center address information:
-------------------------
NASC

Web:..http://nasc.nott.ac.uk
Email.arabidopsis at nottingham.ac.uk
Phone.+44 (0)115 9791216
=46ax...+44 (0)115 9513251
Address:
Dept of Life Science
Nottingham University
Nottingham
NG7 2RD
UK
--------------------------

ABRC

Web:...http://aims.cps.msu.edu/aims
Emfail.arabidopsis+ at osu.edu
Phone:.+01 614-292-9371
=46ax:...+01 614-292-0603
Address:
The Ohio State Univ.
309 B&Z Bldg.
1735 Neil Ave.
Columbus, OH 43210
USA




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