Post Doc in Gene Silencing

Heriberto Cerutti hcerutti at
Wed Jun 10 10:35:18 EST 1998

Two Postdoctoral Positions
Molecular Mechanisms of Gene Silencing

Postdoctoral positions are available immediately to study the molecular
mechanisms responsible for epigenetic transcriptional inactivation.  We
have recently demonstrated that two processes, namely chromatin/nuclear
domains and DNA methylation, might cause the silencing of foreign
transgenes in the unicellular green alga Chlamydomonas reinhardtii
(Plant Cell 9: 925-945 [1997]; Genetics 145: 97-110 [1997]).  To test
these hypotheses, our specific research objectives are directed at
identifying and characterizing some of the gene products responsible for
the inactivation.  We are currently isolating tagged mutants defective
in epigenetic silencing; cloning and characterizing several tagged genes
involved in transcriptional repression (homologous genes will be
isolated in Arabidopsis); and mapping the chromosomal location of
silenced and stable transgenic loci.

We expect that these results will contribute to a greater understanding
of epigenetic phenomena in eukaryotes, including higher plants and
mammals, since the molecular mechanisms appear to be evolutionarily
conserved.  This knowledge, in turn, might be useful for improving
transgenic technology.  Successful candidates will have access to
state-of-the-art facilities in the George W. Beadle Center for Genetics
Research and acquire experience in epigenetics, chromatin, and DNA
methylation analyses.  A background in molecular genetics and
biochemistry would be desirable.

The project is supported by NSF and a Nebraska Research Initiative and
the positions are fully funded for two years with the possibility of
renewal for a third year dependent on satisfactory progress.  Applicants
should send a CV, statement of research experience, and names and
addresses (including e-mail) of three references to:  Dr. Heriberto
Cerutti, School of Biological Sciences, University of Nebraska, E211
Beadle Center, P.O. Box 880666, Lincoln, NE 68588-0666; phone (402)
472-0247; fax (402) 472-8722; e-mail hcerutti at

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