Particle Bombardment

[daemon at net.bio.net]

Hi All,

we just bought a BioRad Helium gun and could do with some technical tips
from experienced users. What particle size (gold or tungsten?) and pressure
discs are recommended for (successfull) bombardment of onion cells or
Arabidopsis leaves?

Regards

Ruediger


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Dr. Ruediger Simon
Institut fuer Entwicklungsbiologie
Universitaet zu Koeln
Gyrhofstr. 17
50923 Koeln
Germany

e-mail: rsimon at novell.biolan.uni-koeln.de
Tel.: +49-221-470-3130
=46ax.: +49-221-470-5164
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