sathish calling from india

sathish at no3.superb.net sathish at no3.superb.net
Wed May 27 21:16:26 EST 1998


Dear Professor
     I am Sathishkumar.R, just submitted Ph.D thesis and looking for
a Post Doctoral fellowship in your esteemed group.I am enclosing my
CV for your kind perusal.I am at your disposal for any clarifications.
Awaiting your reply soon.
Thankingyou.
Sincerely
Sathishkumar.R





*******************************
R.Sathishkumar,M.Sc., M.Phil.,
Research Fellow,
Department of Plant Sciences,
School of Biological Sciences,
Madurai Kamaraj University,
Madurai 625 021,
India.

E-mail : sathish at superb.net
Tel    : 91-452-858214, 858464
Fax    : 91-452-859139, 859105

















Content-Type: TEXT/PLAIN; charset=US-ASCII; name="cv.1"
Content-ID: <Pine.GSO.3.96.980527011128.20827E at no3.superb.net>
Content-Description:

CURRICULUM VITAE
(Updated on 25.3.98)


                                               R .SATHISHKUMAR
                                               Research Fellow
                                               Department of Plant Sciences
                                               School of Biological Sciences
                                               Madurai Kamaraj University
                                               Madurai - 625 021, India
                                               Tel   : +91-452-858214
                                               Fax   : +91-452-859105, 859139
                                               E.mail: sathish at superb.net

===============================================

BIO-INFORMATION

Name in Full  	Ramalingam Sathishkumar

Passport No. 	P216010

Designation  	Senior Research Fellow

Date of birth 	17th May, 1968

Sex  	        Male

Nationality  	Indian

Marital status  Married

Permanent
    address     S/o. Sri. B.Ramalingam
		Pelakuppam Post
		Tindivanam Taluk
	        Villupuram District
		Tamil Nadu , 604002, India
		Phone :  +91-4147-22541

ACADEMIC QUALIFICATIONS

Dec. '97        Doctor of Philosophy (Ph.D.). Thesis Submitted
Thesis
Submitted      "Studies  on the role of  membrane lipids in  in  vitro
		responses and application of cell cultures for DNA
		delivery in rice"

                Madurai Kamaraj University, Madurai

Nov. '91 	Master of Philosophy (M.Phil.)	74.4%

                "Efficacy of Glyphosate on  some photosynthetic
		process of weed species associated with
		Blackgram"

                Pondicherry Central University, Pondicherry

May. '90  	Master of Science (M.Sc.) 68.4%
                (Botany)
		Madras Christian College, Chennai

April '88	Bachelor of Science (B.Sc.) OGPA 5.05/6
		(Botany)                    (Over All Grade Point Average)
		Loyola College, Chennai

HONOURS

1. Awarded first prize in poster presentation at, National Symposium
on "Recent Advances in Biotechnological Applications of Plant
tissue culture and Cell culture" in the Genetic Engineering and
Crop Improvement session, held at Central Food Technological Research
Institute, Mysore, India during June 22 - 24, 1995

2. Awarded third prize in poster competition in"National
Science day celebrations"organised by School of Biotechnology,
Madurai Kamaraj University, Madurai on 28 February 1995

3. Awarded the best poster at National level, during the annual
meeting of the "Society of Biological Chemists"(India)
held at Madurai Kamaraj University, Madurai, India, on Dec 19 - 22,
1994

4. Awarded the best report by Central Pollution Control Board,
India, prepared on "Heavy metal pollution and its effects
on human health and remedial measures" on 4 March, 1991

AWARDS / FELLOWSHIPS

National

Graduate Aptitude Test in 	70 percentile
Engineering (GATE '91)

Junior Research Fellowship	Department of Science and Technology,
                                Govt. of India, funded  project(1992 - 1994)

Senior Research Fellowship	Council of Scientific and Industrial Research,
			 	Govt. of  India (1994 - 1997)

International

Short-term Research 		Deutsch Academic Exchange Service
Fellowship (6 months)		(DAAD), Germany (Aug 1996 - Jan 1997)

MEMBERSHIPS

International 			International Society  for Plant Molecular
				Biology,  Athens, USA

TECHNICAL PROFICIENCY

Plant  Biochemistry

Isolation of  subcellular components  (chloroplasts,  plasma
membrane, etc.,); measurement of photosynthetic electron transport;
extraction and estimation of photosynthetic pigments; biomass estimation;
in vivo CO2 fixation using 14CO2; RUBPcase assay;  extraction and  separation
of membrane lipids using TLC; estimation of various classes of lipids;
extraction of proteins and quantification; starch metabolic pathway enzyme
activity measurements; starch and sugar analysis; determination of TCA
metabolic intermediates

Plant Tissue Culture

Induction and maintenance of calli and suspension cultures;
regeneration of plantlets and hardening to field conditions; plating
of cell suspensions to obtain plantlets through microcalli; protoplast
isolation and culture; immobilisation of  cell and protoplast  cultures

Molecular Biology and Genetic Engineering

Plasmid DNA  extraction and purification; E. coli transformation;
restriction enzyme analysis; Northern blot; direct gene transfer using
particle bombardment system and Agrobacterium mediated gene transfer

RESEARCH EXPERIENCE

Ph. D Programme

GUS gene expression in cell cultures of Indica rice Cv. IR 20

Particle mediated gene delivery:

Optimised the culture conditions for a fine dispersion of
plated cells from suspension culture of  rice, which consisted of
single cells and clusters of 2-4 cells.  These fine suspension  cells
were employed for DNA delivery by microprojectile bombardment system
(Bio-rad, PDS1000/He).  An intron containing plasmid construct (pIG 221)
with GUS-gene under the control of  CMV 35S promoter was employed.The
bombardment conditions for particle delivery process was optimised
by varying,   helium pressure and flight distance for maximum GUS-gene
expression.  The histochemical staining showed maximum of 28% of GUS
gene expression in these cultures.  Also the suspension culture derived
large cell clumps were employed and compared the GUS gene delivery
efficiency.

Agrobacterium mediated gene delivery

The mature seed embryo calli was employed for  transformation
using  A. tumefaciens strain EHA 101 harbouring pIG121 Hm,
a binary vector.  The parameters for maximum GUS gene expression was
optimised. The calli of above 3 months old showed more GUS gene expression
than one month old calli.  The aminoacid during the co-cultivation
found to be vital.  Acetosyringone at 150 uM seems to improve
the GUS gene expression.  The maximum  GUS gene expression was around
30-40%.

Role of membrane lipids  in  in vitro systems of  rice Cv.  Rasi

Changes in lipid composition and growth rate due to growth
factor supplements in  callus cultures of rice

A set of growth factors - choline, putrescine and pectin
were supplemented in rice callus culture media and determined lipid
changes in total callus and in its plasma membrane enriched fraction.  The
growth factor induced lipid changes were correlated with rate of
growth.Choline
supplementation led to increase in relative contents of phosphatidylcholine
(33%) , phosphatidylinositol (60%) and decrease in the relative contents
of phosphatidylethanolamine (30%), phosphatidylserine (25%).  The
growth rate of + choline cultures  significantly  increased by 2.4
fold as compared to control cultures.  Pectin and putrescine supplementation
inhibited the growth in these cultures, were phosphatydicacid contents
increased when compared to control.  In conclusion, choline found
to be ideal growth supplement for inducing fast proliferation of rice
cultures.

Lipid changes during in vitro differentiation of rice cultures

Lipid profile was determined in shoot and root differentiating
callus and compared with undifferentiated callus (control).  Role
of individual lipids species were elucidated.  Significantly phospholipids
and galactolipids was high 29% and 3% respectively in shoot regenerating
regions of callus.

DAAD Short-term research fellowship programme in the lab
of Dr. Richard N.Trethewey, Group Leader,  Max-Planck Institute for Molecular
Plant Physiology, Golm, Germany

Role of Glucose in Plant Gene Expression using Potato Transgenics:

Characterization of gene expression in various transgenic
potato plants affected in their ability to phosphorylate glucose was
undertaken.  To this effect, various experiments like enzyme activity
measurements, determination of TCA cycle intermediates and northern
blot analysis was performed.

M.Phil.  Programme

Efficacy of Glyphosate on some photosynthetic process
of weed species associated with Blackgram

Efficacy of glyphosate varied between the crop and weed species.
Findings provide substantial evidence for the inhibition of the
pigment biosynthesis which lead to all significant changes of reduced
CO2 fixation, photochemical activities and carboxylation activities.
Our findings also suggest that glyphosate may not have significant
primary effects on the photosynthetic reactions, its all only secondary
steps in mode of action.  The differences in the efficacy of glyphosate
on the crop and weed species are attributed to the basic genetic
potentials of the plants in tolerating the herbicide applied
through foliage.

PAPERS PRESENTED IN THE SYMPOSIUM

INTERNATIONAL

1. R.Sathishkumar and K.Manoharan(1997) Changes in the composition
of membrane lipids during in vitro regeneration of rice.
In:International Conference on Fronteirs in Biotechnology,held
in Trivandrum,India during Nov.26-29.1997.

2. R.Sathishkumar, Astrid Blau, Richard Trethewey, Lothar Willmitzer
and K.Manoharan (1997)  Transient gene expression in indica
rice cultures following particle bombardment and Agrobacterium
- mediated DNA delivery.In 5th International Congress of Plant
Molecular Biology, held in Singapore on Sept. 21-27.1997.

NATIONAL

1. R.Sathishkumar and K.Manoharan (1995)  A cell culture
based genetic transformation system for the rice cultivar IR 20 by
employing microprojectile bombardment. In: Symposium on "Recent
Advances in Biotechnological Applications of Plant tissue and Cell
culture and XVIII meeting of Plant Tissue Culture Association of India"
held at Central Food Technological Research Institute, Mysore during
June 22-24.1995

2. R.Sathishkumar and K.Manoharan (1994)  Regulatory role
of membrane lipids in growth and differentiation responses of rice
cultures. In: "Annual meeting of the Society of Biological
Chemists" (India) held at Madurai Kamaraj University, Madurai,
India, during Dec. 19-22, 1994

3. R.Sathishkumar and K.Manoharan (1993)  Growth responses
of callus cultures of rice in relation to membrane lipid composition.
In: Symposium on "Recent developments in Molecular Biology and
Genetic Engineering", held at Tamil Nadu Agricultural University,
Coimbatore, India during Dec. 29-31, 1993

4. N.Muthu Meenakshi, P.Theriappan, R.Sathishkumar,  M.
Ramesh, K.Manoharan, Aditya K. Gupta and A.Gnanam (1993) In vitro
plant regeneration in Arachis hypogaea L. and Glycine
max L. In:  DAE  symposium on "Photosynthesis and Molecular
Biology"  by  Department of Atomic Energy, Govt. of India, held
at Jawaharlal Nehru University, New Delhi, India,  during March 17-19,
1993

5. K.Manoharan, M.Ramesh, R. Sathishkumar, Aditya K. Gupta
and A.Gnanam (1993)  Tissue culture studies on Oryza sativa
L. for induction of callus, plantlet regeneration and cell suspension
cultures. In:  DAE symposium on "Photosynthesis and Molecular
Biology"  by  Department of Atomic Energy, Govt. of India held
at Jawaharlal Nehru University, New Delhi, India during March 17-19,
1993

PUBLICATIONS

In Proceedings

1. R.Sathishkumar and K.Manoharan (1995)  A cell culture
based genetic transformation system for the rice cultivar IR 20 by
employing microprojectile bombardment. In: Proceedings of  symposium
on "Recent Advances in Biotechnological Applications of Plant tissue
and Cell culture and XVIII meeting of Plant Tissue Culture Association
of India" Published by Oxford - IBH publishing Co. Pvt. Ltd., New
Delhi, held at Central Food Technological Research Institute, Mysore
during June 22-24, 1995.

2. N.Muthu Meenakshi, P.Theriappan, R.Sathishkumar,  M.Ramesh,
K.Manoharan, Aditya K. Gupta and A.Gnanam (1993)  In vitro
plant regeneration in Arachis hypogaea L. and Glycine
max L. In:  Proceedings of DAE  symposium on"Photosynthesis
and Molecular Biology"  Published  by  Department of Atomic Energy,
Govt. of India, held at Jawaharlal Nehru University, New Delhi, India,
during March 17-19, 1993.

In refereed journals

1. R.Sathishkumar, Shipra Agrawal and K.Manoharan  (1997/8)
Particle mediated DNA delivery and transient expression of GUS gene
in plated cells of rice.  Biologia Plantarum (Prague) 40(2),pp.305-309

2. R.Sathishkumar and K.Manoharan (1996)  Lipid changes
due to growth factor supplements in callus and plasma membrane - enriched
fraction of rice cultures. Phytochemistry,  Vol.43, No.6, pp.1771-1174


Manuscript in preparation

1. R.Sathishkumar and K.Manoharan, Lipid changes during
in vitro differentiation of rice cultures

2. R.Sathishkumar and A.Ramachandra Reddy.  Efficacy of
Glyphosate on photosynthetic processes of certain weeds associated
with Blackgram


PARTICIPATIONS

National

1. DBT, Government of India sponsored workshop on "Online
information access and  retrieval"  held at Madurai Kamaraj University
during March 26-27, 1996

2. Presented   poster  on "Plant tissue culture" during
National Science Day Celebration organized by School of Biotechnology,
Madurai Kamaraj University, held on Feb. 2, 1996

3. P/ACE seminar on "Capillary electrophoresis" held at
Indian Institute of Science, Bangalore.  Sponsored by Wipro-Biomed
on 2nd December, 1994

4. DBT, Govt. of India, sponsored course on "Computer Applications
in Molecular Biology" held at Pondicherry University during March
29 - April 3,

5. DBT, Govt. of  India,  sponsored short-term training course
on "Structure, function and Molecular Biology of chloroplasts"
held at Madurai Kamaraj University during May 1 - 17, 1991

6. One day symposium on "Recent advances in basic phytology"
held at Madras Christian College, Madras on 16th February, 1989

International

1. Plant Embryogenesis workshop - From Single cell to plant
held at Centre for Applied Molecular Biology, University of Hamburg,
Germany during Sept. 12-14, 1996

REFERENCES

        Dr.K.Manoharan
        Ph.D supervisor

        Permanent

        Reader
        Department of Plant Sciences  Fax: 91 452 89105,859139
        School of Biological Sciences Tel: 91 452 858214
        Madurai Kamaraj University
        Madurai 625 021
        India

        Present

        Visiting Scientist            Fax: 331 290 7015
        C/o Prof. Woo Sung Lee     E.mail:mano at yurim.skku.ac.kr
        Sung kyun Kwan University
        Department of Biology
        Suwan, Kyungi-Do
        440 746, Korea

        Dr.Kailash Paliwal            Fax	: +91-452-859105, 859139
	Professor and Head 	      Tel	: +91-452-858432
	Department of Plant Sciences
	Madurai Kamaraj University
	Madurai - 625 021, India

        Dr.Richard Trethewey	      Fax	: +49-331-977 2301
	Group leader and  host	      Tel	: +49-331-977 2309
	Max-Planck Institute for    E.mail   : trethewey at mpimp-golm.mpg.de
	Molecular Plant Physiology
	Karl Liebnecht - Str. 25
	Haus 20, 14476, Golm,
        Germany

        Prof.Lothar Willmitzer	      Fax	: +49-331-977 2301
	Director	              Tel	: +49-331-977 2300
	Max-Planck Institute for     E.mail  : willmitzer at mpimp-golm.mpg.de
	Molecular Plant Physiology
	Karl Liebnecht - Str. 25
	Haus 20, 14476, Golm,
        Germany


                                                      THE END
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