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labelling RNA

Bernd Weisshaar weisshaa at mpiz-koeln.mpg.de
Thu Nov 5 12:34:42 EST 1998


>Dear colleague,
>
>I am trying to make a radiolabelled probe from Arabidopsis RNA. What I
>am trying to do is to see changes in the expression of putative
>downstream genes in my transgenic Arabidopsis. Thus, I will use a probe
>generated from RNA and probe a dot blot that contains around 20 putative
>genes. I wonder if anybody has a protocol for this. Should I use mRNA
>instead of total RNA? I would be really appreciated if anyone can help
>me. Thanks.
>
>Hong-Gu Kang
>
>hkang at ucla.edu

Dear Hong-Gu,

see:
http://www.wenet.net/~telechem/DNA-Microarray-Protocols/

Best regards, Bernd
_____________________________________________________________________

PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-Linné-Weg 10
50829 Koeln
Germany

Tel. office: +49-221-5062 590   Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
e-mail: weisshaa at mpiz-koeln.mpg.de
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