BAC transformation

[David Chevalier]

Dear all,

I plan to try to transform Arabidopsis with an entire BAC. So, I wonder
to know which protocol could be the best: transformation by
microprojectile bombardment seems to be the best solution, but does
anyone have a protocol  for a stable transformation by microprojectile
bombardment?
I know that in tobacco they succeeded to tansfer an insert of 150kb but
it was with a special BAC called BIBAC. Does anyone already use with
BIBAC for Arabidopsis?

Thanks

chevalier david

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CHEVALIER DAVID
University of Zurich
Institute of Plant Biology
Zollikerstrasse 107
8008 Zurich

phone: + 41 1 634 8251
fax: + 41 1 634 8204
dcheval at botinst.unizh.ch

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