Agro strain GV3101 Chloramphenicol resistance

[Pete Yorgey]

Does anyone have any idea what concentration of chloramphenicol works for
selection with Agro strain GV3101?  The goal is to select for a Cm
resistant vector in the bacteria.  The closest reference I could find
indicated 25 ug/ml, but GV3101 (pMP90) grew fine on that.

Many thanks,

Pete

Pete Yorgey
Dept. of Molecular Biology
Wellman 10
Massachusetts General Hospital
Boston, MA  02114

Phone:  (617) 726-5950
Fax:    (617) 726-5949