Advice?

Mark Holland MAHOLLAND at ssu.edu
Fri Apr 9 11:25:34 EST 1999


I am trying to develop a lab activity for teaching purposes and would like
to solicit advice from the community on the best way to proceed.
My goal is to use PCR to 1) distinguish among two or more A.t. ecotypes, 2)
analyze  patterns of segregation of PCR products among F2 progeny of a
cross between them.
Can anyone suggest a set of primers that will accomplish this?  For
teaching lab purposes, a small number of PCR products that are reliably
distinguishable is preferable.
Thanks in advance for your help.

Mark A. Holland
Department of Biology
Salisbury State University
Salisbury, MD 21801
maholland at ssu.edu




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