expressing Bacillus genes in Arabidopsis

Barbara Moffatt moffatt at sciborg.uwaterloo.ca
Tue Dec 14 12:14:08 EST 1999


Thanks to all who responded to my query about expressing B. subtilis genes
in Arabidopsis. It would appear that this has not been very successful
without substantial changes to the coding sequence so that they reflect
Arabidopsis codon usage.  Below  are the replies and some useful
references.
Barb Moffatt

I have seen a lot of people trying to express genes from gram+ bacteria in
plants and most failed.
Cristoph Benning

****
Have you looked in to the papers that describe the work to engineer the B.
thurengensis crystal toxin gene to be expressed in plants?
For example, Perlak et al, 1991 PNAS 88:3314-3328.

This might give you some feeling as to what you might need to do get a B.
subtilis gene expressed in plants.
B. Kunkel

****
I don't have any experience with expressing Bacillus genes, but I think
that some of the problems encountered in the early work expressing the Bt
toxin may be relevant.  You might want to check out Bt references from the
mid 1980s.

Tom McKnight

****
Its been my impression that bacterial genes that don't have the same codon
usage as plants poorly accumulate. You will, in most cases, need to engineer
in the correct codon usage.

Bob Creelman



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