total protein extraction/and from Arab. cultured cells

Christophe Pical pbio-cpi at pop.net.lu.se
Wed Jul 14 11:19:13 EST 1999



Dawn wrote:

> Does anyone have a reproducible protocol for total protein isolation
> from a small amount of plant tissue?  I am trying to isolate total
> proteins from various plant tissues for a comparative analysis of
> wild-type and a transgenic line using SDS-PAGE and Westerns.

Since someone is asking...
I have tried unsuccessfully to extract total proteins from Arab cultured
cells. I am looking for a protocol that does not involve mortar
grinding, because my samples are 32P-labelled and in addition I want
quantitatively reproducible results.

Christophe




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