total protein extraction/and from Arab. cultured cells
pbio-cpi at pop.net.lu.se
Wed Jul 14 11:19:13 EST 1999
> Does anyone have a reproducible protocol for total protein isolation
> from a small amount of plant tissue? I am trying to isolate total
> proteins from various plant tissues for a comparative analysis of
> wild-type and a transgenic line using SDS-PAGE and Westerns.
Since someone is asking...
I have tried unsuccessfully to extract total proteins from Arab cultured
cells. I am looking for a protocol that does not involve mortar
grinding, because my samples are 32P-labelled and in addition I want
quantitatively reproducible results.
More information about the Arab-gen