total protein extraction
carl.fossdal at nisk.no
Thu Jul 15 11:20:35 EST 1999
This seem to work for me:
Try to boil your sample in 2X SDS sample buffer with 0.2M DTT, grind the
tissue with a toothpick if necessary, centrifuge away the debree and load
onto the gel.
To determine the protein amount spot dilutions on a PVDF membrane and stain
with coomasie, and destain, and compare to a known amount of protein
treated in the same fashion
Carl Fossdal, Aas, Norway
Dawn <paxsond at muohio.edu> skrev i artikkelen <378B5DA4.21A3 at muohio.edu>...
> Does anyone have a reproducible protocol for total protein isolation
> from a small amount of plant tissue? I am trying to isolate total
> proteins from various plant tissues for a comparative analysis of
> wild-type and a transgenic line using SDS-PAGE and Westerns.
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