intercellular wash fluid

[Nicolas Baumberger]

Hi everybody,
Could somebody provide me with a protocol for preparing intercellular
wash fluid (IWF) from arabidopsis seedlings. My goal is to verify the
presence of a protein in the apoplast.
Thank you in advance.
Nicolas



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Nicolas Baumberger
Institute of Plant Biology, University of Zürich
Zollikerstr.107, CH- 8008 ZURICH, Switzerland
Tel: 41-1-6348232
Fax: 41-1-6348257
Email: nbaumber at botinst.unizh.ch


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