intercellular wash fluid

[Jon Monroe]

Hi Nicholas,

Have a look at our paper on apoplastic alpha-glucosidases (Plant
Physiology 1999, 119:385-397
http://www.plantphysiol.org/cgi/content/abstract/119/2/385),
especially figure 11.  We grew mustard seedlings under water for 3
days then gently extracted them with a high salt buffer.  The protein
came out and few cells were broken.  I didn't try it with Arabidopsis
seedlings but I bet it would work.

Good luck.

Jon

>Hi everybody,
>Could somebody provide me with a protocol for preparing intercellular
>wash fluid (IWF) from arabidopsis seedlings. My goal is to verify the
>presence of a protein in the apoplast.
>Thank you in advance.
>Nicolas
>
>
>
>--
>Nicolas Baumberger
>Institute of Plant Biology, University of Z¸rich
>Zollikerstr.107, CH- 8008 ZURICH, Switzerland
>Tel: 41-1-6348232
>Fax: 41-1-6348257
>Email: nbaumber at botinst.unizh.ch
>
>
>---

---------------------------------------------------
 Jonathan D. Monroe           Associate Professor
 Department of Biology       office: 540-568-6649
 MSC 7801                       lab: 540-568-6045
 James Madison University       fax: 540-568-3333
 Harrisonburg, VA 22807   email: monroejd at jmu.edu
 http://csm.jmu.edu/biology/monroejd/jmonroe.html
---------------------------------------------------