Amplification of two-hybrid library

Demetra Elias eliasdemetra at hotmail.com
Mon Sep 27 10:44:13 EST 1999


Hello

I have a two-hybrid lambda cDNA library that is getting old and which I
would like to amplify. The library was amplified originally from 36 million
independent clones so I want to plate 100 million clones to amplify. This
would require 2000 plates if plating 50 000 plaques per plate. I am
considering two things:
      1) to amplify the library by plating with a high density
      2) to amplify the library by growing the phage in liquid
If any one has any suggestions please let me know.

Thank you
Demetra




More information about the Arab-gen mailing list