Plasmid curing method

[Patricia Monteiro]

Dear Colleagues:

I am looking for a good protocol to cure a 50 kb plasmid of a gram negative
phytopathogenic bacterium. In case anyone has it, I will be very thankful.

Thanks a lot in advance.

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Patricia Brant Monteiro
Lab. Biologie Cellulaire et Moléculaire
Institut National de la Recherche Agronomique (INRA)
Centre de Recherches de Bordeaux
71 Avenue Edouard Bourleaux - B.P. 81
33883 Villenave d'Ornon Cedex. France
Tel: (33) 05.56.84.31.51
Fax: (33) 05.56.84.31.59
E-mail: monteiro at bordeaux.inra.fr