Best controls in Western blots (ECL) ?

Laurent VESPA Laurent.VESPA at ujf-grenoble.fr
Wed Apr 12 04:27:34 EST 2000


Hi all,

I would like to quantify my signal in western blots but do not have a
control yet.
So I would like to know what you guys are using as a loading control in
western blots (in my case with ECL detection).
I have heard of beta-tubulin or histone antibodies (commercialy available).
Any experience/advices with these antibodies or others would be appreciated.



Laurent VESPA
Universite Joseph Fourier
CERMO - Plastes et Differenciation Cellulaire
CNRS UMR 5575
B.P. 53
38041 GRENOBLE Cedex 9
Tel: +33 (0) 4 76 51 48 92
Fax: +33 (0) 4 76 51 43 36
e-mail : laurent.vespa at ujf-grenoble.fr


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