Antibiotic concentration

Tadas Panavas panavas at bio.umass.edu
Thu Apr 13 16:45:48 EST 2000


Dear all,

I heard that high antibiotic concentration in the selection medium of
primary transformants selects for Arabidopsis plants that have multiple
T-DNA insertions. My goal is a single T-DNA insertion. I have either Kan or
Hyg resistant lines.

Any suggestions what could be the lowest kanamycin or hygromycin
concentrations in the medium to ensure the selection from untransformed
seedlings.

Tadas

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Tadas Panavas, Ph.D.
Biology Dept.
Morrill Sc. Center
University of Massachusetts,
611 N. Pleasant
Amherst, MA 01003
tel.: (413) 545-9622
fax.: (413) 545-3243


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