Antibiotic concentration

Tadas Panavas panavas at
Thu Apr 13 16:45:48 EST 2000

Dear all,

I heard that high antibiotic concentration in the selection medium of
primary transformants selects for Arabidopsis plants that have multiple
T-DNA insertions. My goal is a single T-DNA insertion. I have either Kan or
Hyg resistant lines.

Any suggestions what could be the lowest kanamycin or hygromycin
concentrations in the medium to ensure the selection from untransformed


Tadas Panavas, Ph.D.
Biology Dept.
Morrill Sc. Center
University of Massachusetts,
611 N. Pleasant
Amherst, MA 01003
tel.: (413) 545-9622
fax.: (413) 545-3243


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