BAC methylation

Chunxin Wang cxwang at wam.umd.edu
Tue Aug 1 13:03:02 EST 2000


Hi, all:

Does anybody know if the BAC DNA is methylated or not? One of my labmates
is trying to subclone one BAC into a binary vector. He cut the BAC with
Cla I, but it does not give rise to the right predicted digestion pattern.
He suspected that some sequences around Cla I sites has been methylated.
Is it true?

Black




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