BAC methylation

Yasuo Niwa niwa at fns1.u-shizuoka-ken.ac.jp
Tue Aug 1 23:59:08 EST 2000


Dear Chunxin Wang,

If you use the Dam+ E. coli,
A of GATC is methylated.
So the Cla I site of gATCGAT will be blocked
by dam methylation.

Yasuo Niwa

>Hi, all:
>
>Does anybody know if the BAC DNA is methylated or not? One of my labmates
>is trying to subclone one BAC into a binary vector. He cut the BAC with
>Cla I, but it does not give rise to the right predicted digestion pattern.
>He suspected that some sequences around Cla I sites has been methylated.
>Is it true?
>
>Black
>
>---
>


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