Problem with Arabidopsis seeds sterilization

BHKim byung-hoon.kim at uni-tuebingen.de
Tue Feb 1 06:12:38 EST 2000


Hello everyone,

I sterilized Arabidopsis seeds which have been transformed by vacuum
infiltration. But one week after growing on the selection plates I found
a lot of fungi on the plates. I repeated it again and again, but I
couldn't get rid of them.
The sterilization methode was :
       3000 - 4000 seeds per 1.5 ml Epp. tube
       shaking for 5 min, RT, in (95% EtOH and 0.1% Triton X-100)
       shaking for 5 min, RT, in (Potassium chloride solution with one
drop of Tween 20)
       washing 3 times with sterile H2O

Could anyone out there give me a tip?
Thanks in advance for any comment

Byung-Hoon Kim


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