Problem with Arabidopsis seeds sterilization
sukwhan at U.Arizona.EDU
Tue Feb 1 16:40:48 EST 2000
Hi Byung-hoon Kim, I have same experience with you. Something on your
plate after sterilization might be agrobacteria kept in seed coat.
Hygromysin work to kill them in my experiment.
On 1 Feb 2000, BHKim wrote:
> Hello everyone,
> I sterilized Arabidopsis seeds which have been transformed by vacuum
> infiltration. But one week after growing on the selection plates I found
> a lot of fungi on the plates. I repeated it again and again, but I
> couldn't get rid of them.
> The sterilization methode was :
> 3000 - 4000 seeds per 1.5 ml Epp. tube
> shaking for 5 min, RT, in (95% EtOH and 0.1% Triton X-100)
> shaking for 5 min, RT, in (Potassium chloride solution with one
> drop of Tween 20)
> washing 3 times with sterile H2O
> Could anyone out there give me a tip?
> Thanks in advance for any comment
> Byung-Hoon Kim
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