expression in E. coli

Michael Prigge prigg007 at prigg007.email.umn.edu
Fri Feb 4 16:31:46 EST 2000


In article <01JLCSR43H7M003BQC at vax2.concordia.ca>,
JOYCEP at vax2.concordia.ca (Paul Joyce) wrote:

>  There are a number of plasmids designed containing the appropriate tRNA genes
>  for expression in E. coli.  You might start with pSBETa, b, and c from a
>  Biotechniques paper by Steinbiss et al. volume 19, pages 196-200.  I think
>  that was published in 1995.  Hope that this helps.  Paul Joyce
>  ---

Another possibility is using a tRNA over-expressing strain.  I saw an
ad that one was available from Invitrogen or Gibco BRL--I can't
remember exactly which company sent me that ad.  You should be able to
check their web sites.
Mike





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