Transient Expression

Spencer, S. sps101 at york.ac.uk
Wed Feb 16 09:04:36 EST 2000


I am looking into doing transient expression in arabidopsis (and
tobacco) protoplasts.  My expression plasmid is 3.5 kb.  Would I get
away with using an insert gene of 2.7 kb, making a total final plasmid
size of approx. 6.25 kb, or is this too large for efficient
transformation?

Thanks,

Steven Spencer

---





More information about the Arab-gen mailing list