Transient Expression

Spencer, S. sps101 at
Wed Feb 16 09:04:36 EST 2000

I am looking into doing transient expression in arabidopsis (and
tobacco) protoplasts.  My expression plasmid is 3.5 kb.  Would I get
away with using an insert gene of 2.7 kb, making a total final plasmid
size of approx. 6.25 kb, or is this too large for efficient


Steven Spencer


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