Gene silencing

Julian I. Schroeder julian at
Wed Feb 16 21:06:46 EST 2000

Hello readers,
I am planing to construct a T-DNA vector to clone and express sense and
antisense DNA in the same vector for the purpose of gene silencing. The
promoters that I plan to use are the world famous P35S-CaMv for both
inserts. My question is would the presence of two independent, but
identical promoters cause any complication in the plant transformation
such as recombination, rearrangements etc.??
Please send suggestions to mghassem at
Thank you
Majid Ghassemian
UCSD, Muir Biology


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