Protoplasts & chloroplasts.

Yasuo Niwa niwa at
Fri Feb 25 02:11:56 EST 2000

Dear Dr. Spencer,

At least in Maize, the answer is yes.
Please check the following papers.

C.-C. Chang, J. Sheen, M. Bligny, Y. Niwa, S. Lerbs-Mache, and D. B.
Stern :  Functional analysis of two Maize cDNAs encoding T7-like RNA
polymerases. Plant Cell  (1999) 11 : 911-926.

W-I. Chiu, Y. Niwa, W. Zeng, T. Hirano, H. Kobayashi, and J. Sheen :
Engineered GFP as a vital reporter in plants. Current Biology (1996)
6, 325-330

Yasuo Niwa

>I've been looking into possible controls for use in a transient
>expression experiment I'm planning to do in protoplasts.  It was
>suggested to me to use the small RUBISCO subunit as this would be
>targeted to the chloroplasts (the protein would be tagged).  However, I
>have read that the chloroplasts stops synthesising their own proteins in
>the protoplast.  My question is, does anyone know if the chloroplasts is
>still able to accept proteins when the cell is in the protoplast form?
>The small RUBISCO subunit is usually encoded by the nucleus, and the
>protein is then targetted to the chloroplasts.  Is this still the case
>in protoplasts?  Or will the protein remain in the cytoplasm?  Whether
>this is the case, does anyone know any references saying a positive yes
>or no?  I have been unable to find any.
>Thanks a lot.
>Steven Spencer

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