single colony PCR
Godfrey.Neutelings at univ-lille1.fr
Tue Jan 25 10:37:29 EST 2000
dans l'article firstname.lastname@example.org, "Peter L. Schuerman" à
schuermanp at missouri.edu a écrit le 14/01/00 23:00 :
> Can anyone recommend a good method for single-colony PCR that is more
> elaborate than simply using cells directly in the reaction? In
> particular, I'd be interested in a method that works for
> Agrobacterium. In my experience directly adding cells works well for
> E.coli but I have been getting poor amplification and inconsistent
> results when I have tried this with Agrobacterium. Any help would be
> greatly appreciated.
> Peter L. Schuerman
Simply pick a single colony and place it in 50 microL of water. Boil for 2
min, place on ice. 5 microL is enough for a 50 microL PCR reaction.
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