Transient Expression in Protoplasts

Rik van Gorsel rvgorsel at mpc186.mpibpc.gwdg.de
Thu Jan 27 04:01:25 EST 2000


"Spencer, S." wrote:
>  however, ifanyone has a good protocol for making arabidopsis
>  protoplasts, I would appreciate your advice.

The protocol described by Albrecht von Arnhim in the Course Manual
(still viewable online at http://www.arabidopsis.org/cshl-course/)
of the 1996 Cold Spring Harbor Arabidopsis course worked for me
the first time around (test a range of [mannitol] from 0.3- 0.5 M)

Rik van Gorsel
rvgorsel at mpc186.mpibpc.gwdg.de
---





More information about the Arab-gen mailing list