Transient Expression in Protoplasts

Rik van Gorsel rvgorsel at
Thu Jan 27 04:01:25 EST 2000

"Spencer, S." wrote:
>  however, ifanyone has a good protocol for making arabidopsis
>  protoplasts, I would appreciate your advice.

The protocol described by Albrecht von Arnhim in the Course Manual
(still viewable online at
of the 1996 Cold Spring Harbor Arabidopsis course worked for me
the first time around (test a range of [mannitol] from 0.3- 0.5 M)

Rik van Gorsel
rvgorsel at

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