His-tagging proteins expressed in plants

Thomas Leustek leustek at AESOP.RUTGERS.EDU
Fri Jul 21 15:20:31 EST 2000


<x-flowed>Dear All,

Has anyone ever tried His-Tagging a protein for expression in transgenic
Arabidopsis?

I am interested in using the His-Tag for immunocytological localization
experiments with anti-His-tag antibodies.

I would also like to use the His-Tag as a means to affinity purify the
protein. (Killing two birds with one stone)

Any comments would be appreciated.

Sincerely,
Tom Leustek

Thomas Leustek
Associate Professor
Rutgers University
Biotechnology Center for Agriculture and the Environment
59 Dudley Road
New Brunswick, New Jersey 08901-8520
Telephone: (732)932-8165, extension 326
Fax: (732)932-0312
email: leustek at aesop.rutgers.edu
http://aesop.rutgers.edu/~biotech/faculty/leustek.html


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