His-tagging proteins expressed in plants

Julie Stone stone at frodo.mgh.harvard.edu
Wed Jul 26 10:41:43 EST 2000


A note of warning regarding the His tag in the pCAMBIA vectors.......the
NheI site is not unique.


>To: arab-gen at net.bio.net
>Newsgroups: bionet.genome.arabidopsis
>From: hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury")
>Subject: Re: His-tagging proteins expressed in plants
>Date: 25 Jul 2000 03:07:31 +0100
>Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
>Sender: owner-arab-gen at hgmp.mrc.ac.uk
>Precedence: bulk
>
>The recent collection of the pCAMBIA series of vectors seem suitable for
>something like this. I have not come across any paper describing the
>expression (then again, I have not been looking for such things), but a few
>of these CAMBIA vectors also come with C-terminal His tags. We are planning
>to get the whole set. Should be interesting...
>
>At 09:20 PM 7/21/00 +0100, Thomas Leustek wrote:
>><x-flowed>Dear All,
>>
>>Has anyone ever tried His-Tagging a protein for expression in transgenic
>>Arabidopsis?
>>
>>I am interested in using the His-Tag for immunocytological localization
>>experiments with anti-His-tag antibodies.
>>
>>I would also like to use the His-Tag as a means to affinity purify the
>>protein. (Killing two birds with one stone)
>>
>>Any comments would be appreciated.
>>
>>Sincerely,
>>Tom Leustek
>>
>>Thomas Leustek
>>Associate Professor
>>Rutgers University
>>Biotechnology Center for Agriculture and the Environment
>>59 Dudley Road
>>New Brunswick, New Jersey 08901-8520
>>Telephone: (732)932-8165, extension 326
>>Fax: (732)932-0312
>>email: leustek at aesop.rutgers.edu
>>http://aesop.rutgers.edu/~biotech/faculty/leustek.html
>>
>>
>>---
>>
>></x-flowed>
>>
>>
>>
>>
>Dr. Hiranya Sankar Roychowdhury
>Dept. of Molecular Biology
>PO Box 30001 - 3MLS
>New Mexico State University
>Las Cruces, NM 88003
>
>Lab: (505) 646 4722
>Office: (505) 646 8256
>hroychow at nmsu.edu
>
>
>---
>

Julie M. Stone, Ph.D.
Massachusetts General Hospital
Department of Molecular Biology
50 Blossom St., Wellman 10
Boston,  MA  02114

phone:(617)726-5950
FAX: (617)726-5949
stone at frodo.mgh.harvard.edu



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