Ecoli contamination in Abgrobact.culture

joel.kreps at joel.kreps at
Mon Jul 31 17:21:43 EST 2000

Dear Immo,

Another method is to grow the bacteria with sucrose as the only carbon
source, Agrobacterium can use sucrose but E coli cannot; Agrobacterium can
grow at 37C but cannot maintain its plasmids at that temperature so growth
with antibiotics at 37C could distinguish E coli from Agrobacterium.


Joel A. Kreps
Senior Staff Scientist, Plant Health
Novartis Agricultural Discovery  Institute, Inc.
3115 Merryfield Row, Room 269
San Diego, CA 92121-1125 USA
Phone:  858-812-1071
Fax: 858-812-1105
joel.kreps at

Dear Immo Junghaertchen,

E coli makes a single colony just for over night,
but Agrobacterium takes 2-3 days to make it.

Or Agrobacterium can not grow at 37C.

Yasuo Niwa

>I suspect  E coli contamination in agrobacterium culture. Does anyone
>know of a quick way to distinguish between them? Under the microscope,
>both look like motile, gram negative rods of similar size!
>nr7654 at



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