Sequence of T-DNA insertions?

Petra Epple pepple at email.unc.edu
Tue Mar 28 17:33:16 EST 2000


Hello everybody,

I isolated a few mutants in the Feldmann lines and would like to determine
the number of insertions by Southern blots using the Kan-gene as a probe.
Does anybody know whether the sequence of the T-DNA construct that was
used to generate the mutant lines is available? Alternatively, is there a
list of enzymes which cut the T-DNA only once?

Thanks in advance

Petra


Petra Epple
pepple at email.unc.edu

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