Release of the IMA Ds Insertion lines

Sean May [NASC] sean_may at thale.life.nottingham.ac.uk
Fri May 5 07:07:10 EST 2000


We are pleased to announce the release of the IMA Ds Insertion lines
generated by Prof. Sundaresan and collegues from the National University of
Singapore and the associated flanking sequences to match these germplasm
lines.

These lines contain (mostly) single copy insertions of modified maize
transposable Dissociation (Ds) elements within the Landsberg ecotype.

Each Ds element carries a kanamycin selectable marker and is stably
integrated. The elements are capable of remobilization if the Ac transposase
is provided, e.g., by crossing with one of the Ac starter lines (CS/N8043 -
8049).

These Ds elements also carry the GUS reporter gene designed to act as an
gene trap (SGT or GT) or enhancer trap (SET).

For details of the Ds elements and the transposon mutagenesis system please
see:  Sundaresan, V., Springer, P., Volpe, T., Haward, S., Jones, J.D.,
Dean, C., Ma, H., and Martienssen, R. (1995). Patterns of gene action in
plant development revealed by enhancer trap and gene trap transposable
elements. Genes Dev. 9, 1797-1810

Regions flanking the Ds insertions for the transposant lines were amplified
using TAIL-PCR (Liu et al., 1995). The ensuing sequences were then analyzed
by BLAST searches in two public databases: NCBI GenBank and Arabidopsis
GenBank in Stanford.

Information relating to the flanking regions sequences have been deposited
in the 'Arabidopsis Genome Resource' (AGR) at [ http://ukcrop.net ] and in
'The Arabidopsis Information Resource' (TAIR) at
  [ http://www.arabidopsis.org ], as well as being available directly through
the stockcentre ordering pages

Stocks can be ordered in the usual way from the NASC
(http://nasc.nott.ac.uk) web site.

Individual lines (see http://nasc.nott.ac.uk/ima.html ) may be ordered as
part of the normal user fee system described on our catalogue website.

These lines have a materials transfer agreement associated with them.
Please read the brief instructions carefully before filling out the MTA to
save time later. You will be reminded of the need for an MTA and
instructions will be easily available as you browse and order the stocks.

Sean_May at nasc.nott.ac.uk
Director, Nottingham Arabidopsis Stock Centre (NASC)
University Park, Nottingham University. U.K. NG7 2RD.
FON: +44 (0)115 9513237
FAX: +44 (0)115 9513297
Catalogue: http://nasc.nott.ac.uk
Genomics: http://ukcrop.net
Transcriptomics: http://www.york.ac.uk/res/garnet/garnet.htm


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