Clean RNA from dirty roots...

reineroa at pt.cyanamid.com reineroa at pt.cyanamid.com
Thu Nov 30 20:51:08 EST 2000


You might want to try Ambion 'plant  RNA isolation aid' solution (cat # 9690).
You can add the solution to the lysis buffer in the Ambion RNAqueous kit or to
the lysis buffer in the Qiagen RNeasy kit. It works great!.

Regards

Antonio Reinero
Biotechnology
BASF

____________________Reply Separator____________________
Subject:    Clean RNA from dirty roots...
Author: jshaw at rsbs.anu.edu.au
Date:       11/28/2000 7:21 PM

Hello All,

Judging by what I've found in the archives of this list, this subject
might be an old one but I still haven't found any successful methods.

My problem is that I have to work with soil grown plants which produce a
lot of mucilage, which in turn binds a lot of soil.  Scrubbing of the
roots is not an option in this case and, having tried various kits, the
result is either a pellet with a large amount of brown gunge with it or
no RNA recovered at all (I suspect because it was bound to the brown
gunge that some methods get rid of).

So, my question is, does anybody have a reliable method for getting good
quality RNA out of dirty plant roots to yield a nice, clean prep?

All suggestions welcomed.

Thanks in advance,
John

--
Dr John Shaw
Environmental Biology Group
Research School of Biological Sciences
Australian National University
GPO Box 475
Canberra
ACT 2601
Australia

Tel.   +61 2 6279 8144
Fax    +61 2 6249 4919
E-mail jshaw at rsbs.anu.edu.au



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