Alternaria infection

Guru Jagadeeswaran jagadeesa at hotmail.com
Sat Aug 18 17:34:38 EST 2001


Thanks a lot Dr. Thomma, for your information. Thats very helpful indeed.
Guru Jag
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>From: "Thomma, Bart" <Bart.Thomma at AGR.KULEUVEN.AC.BE>
>To: "Guru Jagadeeswaran" <jagadeesa at hotmail.com>
>Subject: RE: Alternaria infection
>Date: Fri, 17 Aug 2001 21:36:31 +0200
>
>Hi Guru Jag,
>
>In our lab we often do Alternaria infections on Arabidopsis. We almost
>always drop-inoculate because Alternaria will only cause small
>non-expanding lesions on wild-type plants. If you spray-inoculate
>wild-type plants you will get pin=point lesions that are hard to
>observe, if you drop-inoculate you will see a lesion with a diameter of
>a few millimeters, depending on the size of the drop. With the strain we
>use, a lesion will appear between 48 and 72 hours.
>Hope this helps,
>Bart
>
>Dr. Bart Thomma
>Centre of Microbial and Plant Genetics
>Katholieke Universiteit Leuven
>Kasteelpark Arenberg 20
>3001 Heverlee-Leuven
>Belgium
>Tel.: +32-16-32.96.58
>Fax: +32-16-32.19.66
>
>-----Original Message-----
>From:	"Guru Jagadeeswaran"
>Sent:	Thu 8/16/2001 6:00 PM
>To:	arab-gen at net.bio.net
>Cc:
>Subject:	Alternaria infection
>
>Hi all,
>
>We will be inoculating Arabidopsis plants with Alternaria for one of our
>experiments. Which is a better way to infect Arabidopsis plants with
>Alternaria brassicicola: Spraying with a spore suspension OR
>spotting them on leaves a droplets. Is there a reason why most use
>spotting
>rather than spraying- Any response appreciated. Thanks
>
>Guru Jag
>
>




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