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James Meservy mesejl at uleth.ca
Wed Dec 5 16:30:32 EST 2001


I have an inquiry about antibiotic selection in Agrobacterium.
I have inherited a strain of agro that seems to not be sensitive to
streptomycin (50mg/ml) on YEP plates.  Is this lack of selection due to:
1) too low of a streptomycin concentration?
2) the agro being contamination with a plasmid that has a streptomycin
resistance gene?
3) streptomycin not being a selective agent in Agro? and is so,  should I
be using Spectinomycin for my Agro selection.
Any insight would be appreciated.


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