single cells

Sandy L. Berry-Lowe slblowe at brain.uccs.edu
Thu Jan 18 18:23:16 EST 2001


Hi all,
	I would like to get single cells from a cell
culture. I don't want protoplasts but I also don't
want clumps, so I don't think filters/mesh would be
appropriate. Most protoplast procedures call for a mix
of pectinase and cellulase. Has anyone tried using just pectinase?
Any thoughts or comments would be appreciated.

Thanks in advance

sandy berry-lowe
univ. colorado at colorado spring
slblowe at mail.uccs.edu




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