Posdoctoral position open

Pierre Berthomieu berthom at ensam.inra.fr
Tue Jan 30 04:05:19 EST 2001

Post-doctoral position open

	The position is open in the "STRESS group" that is part of a lab
that shows strong expertise in plant mineral nutrition (Drs. Briat,
Davidian, Gaymard, Gojon, Grignon, Sentenac, Touraine...). In the group,
we are interested in functions that control sodium transport and
accumulation in planta. We already characterized a mutant
over-accumulating sodium in the shoot (sas1) as being impaired in a root
function involved in the control of radial transport of sodium from the
soil to the xylem vessels (Nublat et al., 2001, Plant Cell, in press in
the January issue). The positional cloning of this locus is nearly
completed (manuscript in preparation). Other recent publications of the
group are: Cellier et al (1998) Plant Physiol. 116:319; Sarda et al (1999)
Plant Mol. Biol. 40:179; Cellier et al (2000) J. Exp. Bot. 51:299.

	For the post-doctoral position, the research program will consist
in characterizing a second mutant that overaccumulates sodium in the shoot
and displays increased sensitivity to salt stress, the sas2 mutant. The
positional cloning of the sas2 locus has been initiated. At the moment,
the sas2 locus is bordered by two genetic markers (CAPS markers) that are
separated by 270 kb on the physical map. A very good candidate gene lies
in the genomic region delimited by these markers. The first part of work
will consist in completing the positional cloning of the sas2 locus by
performing genetic complementation of the mutant with the wild-type
candidate gene. At the same time, the fine mapping of the sas2 locus and
subsequent subcloning of the region encompassing it will be pursued to
succeed in cloning the sas2 locus even if the candidate gene does not
correspond to the sas2 locus. Then, a molecular analysis will be conducted
to characterize the spatial and temporal expression of the SAS2 gene, in
normal and salt stress conditions. Depending on the progress of the above
mentioned work, physiological analyses of the sas2 mutant will be
conducted, first to characterize the specificity of ion accumulation, the
spatial and temporal pattern of Na+ accumulation, then to study different
aspects of salt tolerance.

	The lab is in Montpellier, south of France, 10 km to the
mediterranean sea. Montpellier is a town with 250,000 people of which
60,000 are University students. Sunny weather almost all year long.

	1 year renewable 1 more year, starting as soon as possible.
	Salary is around 9,500 FF/month net of social and medical charges

	The candidate must have a Ph.D. A very good skill in molecular
biology is requested. A fair experience in plant physiology will be
appreciated but is not prerequisite.
	The candidate can not be of french nationality

	Please submit a letter of application, a curriculum vitae, list of
publications and the names and addresses of three references. Please
indicate when you are ready to start.
	Send your application to
    Dr. Pierre BERTHOMIEU                  e-mail: berthom at ensam.inra.fr
    Assistant Professor                    tel: (+33) 499 612 611
    UMR 5004                               fax: (+33) 467 525 737
    Agro.M   -  Place Viala
    34060 MONTPELLIER Cedex 1

	There is no dead line, candidacies will be examinated as soon as
we receive them.


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