how to study cell-cycle regulated gene

Chunxin Wang cxwang at wam.umd.edu
Wed Jan 31 12:06:29 EST 2001


Dear all:

I'm trying to investigate the regulation of my gene during cell cycle to
test: 1) if the gene is regulated by cell cycle; 2) if it is, then at what
stage the gene is turn on and turn off.

Is there any reference about synchronizable arabidopsis cell suspension or
anybody happens to have it? Is there any alternative way instead of using
the synchroinzed cells, such as transform my gene fused with GUS or GFP
into the wild type or mutant plants and examine the expression of the
gene in the cells at root tip?

Your suggestion is highly appreciated.

Black





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