Gentamycin selection of Arabidopsis seeds - What

Yulan Cheng yulanc at waksman.rutgers.edu
Mon Jul 23 20:47:18 EST 2001


Hi, all,

If anyone has any experience on the selection of transformants (Vacuum
infiltration transformation) by gentamycin, please give me a help.  I am
using plant transformation vector pPZP222 and I have tried up to 100
ug/ml  gentamycin, and the plates are still covered with green and
healthy seedlings.


Thanks  in advance!

Yulan


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