Gentamycin selection of Arabidopsis seeds - What
ligeng.ma at yale.edu
Thu Jul 26 17:46:18 EST 2001
Pls try to use 200-300ug/ml, even 400ug/ml. I have ever tested At by this
concertrations. Try! Further discussion will be welcomed.
My email: kangdingming at yahoo.com or kdm at pku.edu.cn
Yulan Cheng wrote:
> Hi, all,
> If anyone has any experience on the selection of transformants (Vacuum
> infiltration transformation) by gentamycin, please give me a help. I am
> using plant transformation vector pPZP222 and I have tried up to 100
> ug/ml gentamycin, and the plates are still covered with green and
> healthy seedlings.
> Thanks in advance!
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