Gentamycin selection of Arabidopsis seeds - What

ligeng ligeng.ma at yale.edu
Thu Jul 26 17:46:18 EST 2001


Pls try to use 200-300ug/ml, even 400ug/ml. I have ever tested At by this
concertrations. Try! Further discussion will be welcomed.

My email: kangdingming at yahoo.com or kdm at pku.edu.cn

Yulan Cheng wrote:

>  Hi, all,
>
>  If anyone has any experience on the selection of transformants (Vacuum
>  infiltration transformation) by gentamycin, please give me a help.  I am
>  using plant transformation vector pPZP222 and I have tried up to 100
>  ug/ml  gentamycin, and the plates are still covered with green and
>  healthy seedlings.
>
>  Thanks  in advance!
>
>  Yulan
>
>  ---




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