New pooled DNA from T-DNA populations at ABRC

Randy Scholl scholl.1 at osu.edu
Tue Jul 31 14:29:27 EST 2001


<x-flowed>The Arabidopsis Biological Resource Center is pleased to announce
that DNA pools for reverse genetic PCR screening are now available
for 17,000 lines of the Weigel T-DNA population and an additional
5,000 of the Jack T-DNA population.  The screening process is
organized as for our previous populations.  DNA representing
superpools of ca. 1000 lines each is sent initially. This is followed
by two dimension pools, ranging in size from 50 to 200 lines per
pool, depending on the organization of the specific population.  The
smallest unit of pooling, from which the large pools are developed
and which are sent in the final stage as seeds for isolation of the
correct line, are composed of differing numbers of lines ranging from
9 to 20.  Details of the substructure of each superpool is provided
with the DNA samples, and information is also included with the stock
details in TAIR.  All stocks may ordered through the TAIR ordering
system (http://arabidopsis.org). Please direct questions to
dnastock at arabidopsis.org.

The available superpool sets are:


CD10-A: DNA isolated from T-DNA lines donated by From D. Weigel; set
of 17 superpools (cs. 1000 lines/pool) for primary screening; stock
includes primers and DNAs; price of stock includes followup screening
including secondary pools and associated seed pools for any positive
results; each superpool sample contains 40 microliters of an aqueous
solution of DNA in TE; the total concentrations of DNA in each
superpool tube range from 45 to 68 ng/microliter, with each line
contributing equally to the final concentration.  Price: $175.

CD7-7: DNA isolated from T-DNA lines donated by T. Jack; second set
of 5 superpools DNA  (1000 lines/pool); stock includes primers and
DNAs; price of stock includes followup screening including secondary
pools and associated seed pools for any positive results; each
superpool sample contains 40 microliters of an aqueous solution of
DNA in TE; the total concentration of DNA in each superpool tube is
50ng/microliter with each line contributing equally to the final
concentration.  Price: $50.

All stocks may be ordered through TAIR (http://arabidopsis.org).

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