Old question : RNA primer extension on ABI377

Moez Torki torki at sakura.cc.tsukuba.ac.jp
Wed Jun 13 20:54:41 EST 2001


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ABI377</title></head><body>
<div>I could see many people on methds-reagnts Newsgroup and elsewhere
looking for protocols to perform non-RI transcription start mapping on
an automated sequencer, but no answer since 1997...</div>
<div>Recently I found this protocol on the web :
http://bio.licor.com/App_513/App513.htm describing a method for the
LI-COR 4000L apparatus. Did anyone perform similar experiment on
ABI377. I'm a new user for this this machine and I would like to know
(1) what should be the appropriate label for the primer (2) would it
be possible to run the extended products together with a Dye
Terminated sequences (3) what are the appropriate settings on the
machine... ? I am quiet confident that primer extension can be
performed on ABI377.</div>
<div>Thanks for any input.</div>
<div><br></div>
<div>Moez</div>
<div><br></div>
<div><font color="#000000">Moez TORKI, Eng., Ph.D.</font></div>
<div><font color="#000000">University of Tsukuba,<br>
Institute of Agriculture and Forestry,<br>
Tsukuba, Ibaraki 305-8572, JAPAN</font></div>
<div><font color="#000000"><br>
+81 (0) 298 53 7262 / +81 (0) 298 53 4710 (voice)<br>
+81 (0) 298 53 6617 (fax)</font></div>
<div><font color="#000000">torki at sakura.cc.tsukuba.ac.jp
(email)</font></div>
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