Fast neutron irradiation and cloning

Rachel Green rmgreen at ucla.edu
Wed Jun 20 13:49:31 EST 2001


<x-flowed>We have two questions about our flowering -time mutants that were
generated by fast neutron irradiation (at the IAEA in Vienna).
1. What is known about the size of deletions produced in Arabidopsis
following fast neutron irradiation?
2. Can the larger deletions caused by fast neutron irradiation be
used as a basis for cloning the mutations eg by subtractive
hybridization. Is there anyone who has tried this?

Thanks

Rachel Green
--------------------------------
Dr. Rachel Green
Department of MCD Biology
UCLA
Box 951606
LA CA 90095-1606

Phone	310/825-0130
Fax	310/206-4386
email rmgreen at ucla.edu

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